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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has been noticed in many tumors, which results in chromosome instability, and thus could contribute to tumorigenesis. This protein was uncovered to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a essential position in marketing mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are explained. Two added splice variants have been claimed but in depth nucleotide sequence information just isn't yet accessible.

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Blocking peptides are peptides that bind especially for the goal antibody and block antibody binding. These peptide commonly has the epitope acknowledged with the antibody.

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This guideline outlines additional feasible optimization of cellular and immunoassay parameters to be sure the very best success are...

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This manual outlines further attainable optimization of cellular and immunoassay parameters to guarantee the very best success are...

Antibodies certain to the blocking peptide no more bind to your epitope within the concentrate on protein. By comparing the staining from the blocked antibody vs . the antibody on your own, one can see which staining is particular.

Goal Information and facts Cyclin E belongs to the hugely conserved cyclin spouse and children, whose users are characterised by a remarkable periodicity in protein abundance with the cell cycle. Cyclins functionality here as regulators of CDK kinases. Unique cyclins show distinct expression and degradation styles which contribute on the temporal coordination of every mitotic celebration. Cyclin E types a complex with and capabilities to be a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S changeover. This protein accumulates in the G1-S period boundary and it is degraded as cells progress by way of S section.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both antibodies...

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